Nasdaq said Biogen, CDW Corporation, GlobalFoundries, Lululemon Athletica, On Semiconductor and Trade Desk were removed from the technology-heavy index.
New entrants included Alnylam Pharmaceuticals, Ferrovial, Insmed, Monolithic Power Systems, Seagate Technology and Western Digital.
Strategy started out as software company, MicroStrategy, but pivoted to bitcoin investing in 2020. It was included in the Nasdaq 100 last December under the index's technology sub-category.
Global index provider MSCI has also raised concerns about the presence of digital-asset treasury companies in its benchmarks. MSCI is due to decide in January whether to exclude Strategy and similar companies.
The Nasdaq changes are expected to take effect on December 22. The Nasdaq 100 index tracks the largest non-financial companies by market capitalization listed on the exchange.
